论文部分内容阅读
本研究通过对我国部分地区的自然人群和非甲非戊型肝炎病人进行HGV和TTV感染的分子流行病学研究 ,探讨这两种病毒在我国肝炎发病尤其是在非甲非戊型肝炎中的作用和地位。用建立的PCR方法检测血清标本中的HGVRNA和TTVDNA ,对调查的自然人群和非甲非戊型肝炎病人血清标本进行检测。HGVRNA采用反转录PCR(RT -PCR)检测 ,TTVDNA则采用巢式PCR方法检测。结果表明 ,HGV在自然人群中HGVRNA携带率为 0 6 %～ 1 1% ,TTV的病原携带率则高达 7 1%～ 12 4 % ;非甲非戊型肝炎病人中HGV和TTV的阳性率分别为 7 9%和 2 8 1%。在所检测的非甲非戊肝炎病人中HGV和TTV的总感染率为 35 9% (包括了HGV和TTV的混合感染 )。因此 ,HGV在自然人群中感染率低 ,而且在非甲非戊型肝炎病人中约为 10 %的病人是由HGV的感染所致 ,HGV不是非甲非戊型肝炎病人的主要病因。TTVDNA在自然人群中的携带率约为 10 % ,类似于HBVDNA的携带率。虽然在非甲非戊型肝炎病人中TTVDNA的阳性率为 2 8% ,但仍然有高达 6 0 %的病人病因不明 ,TTV感染也不是非甲非戊型肝炎病人的主要致病病原。
In this study, molecular epidemiology of HGV and TTV infection in natural populations and non-A, non-A, and E hepatitis patients in some areas of our country was conducted to investigate the relationship between these two viruses in our country with hepatitis, especially in non-A, non- Role and status. The serum samples were tested for HGVRNA and TTVDNA using the established PCR method, and the serum samples of the investigated natural population and non-A non-E hepatitis patients were detected. HGVRNA was detected by RT-PCR and TTVDNA by nested PCR. The results showed that the prevalence of HGVRNA in HGV was 0 6% -1 1% in natural population and that of TTV was 71% ~ 124%. The positive rates of HGV and TTV in non-A non-E hepatitis were 7 9% and 2 8 1% respectively. The overall prevalence of HGV and TTV among non-AETA patients tested was 35.9% (including a mixed infection of HGV and TTV). Therefore, the prevalence of HGV infection in the general population is low, and about 10% of non-A, non-A, E caused by HGV infection is not the major cause of non-A non-E hepatitis. The carrying rate of TTVDNA in the natural population is about 10%, similar to the carrying rate of HBVDNA. Although the positive rate of TTVDNA in non-A, non-E hepatitis patients is 28%, up to 60% of the patients still have etiological factors unknown and TTV infection is not the major causative agent of non-A non-E hepatitis patients.