A study was conducted to determine the genetic diversity of 39 determinate and indeterminate tomato inbred lines collected from China,Japan,S. Korea,and USA. Using 35 SSR polymorphic markers,a total of 150 alleles were found with moderate levels of diversity,and a high number of unique alleles existing in these tomato lines. The mean number of alleles per locus was 4.3 and the average poly-morphism information content (PIC) was 0.31. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) clustering at genetic similarity value of 0.85 grouped the inbred lines into four groups,where one USA cultivar formed a separate and more distant cluster. The most similar inbred lines are from USA,both with determinate type,whereas the most different lines are from USA (Us-16) and Japan (Ja-2) with determinate and indeterminate growth habit,respectively. Clustering was consistent with the known information regarding geographical location and growth habit. The genetic distance information reported in this study might be used by breeders when planning future crosses among these inbred lines. A study was conducted to determine the genetic diversity of 39 determinate and indeterminate tomato inbred lines collected from China, Japan, S. Korea, and USA. Using 35 SSR polymorphic markers, a total of 150 alleles were found with moderate levels of diversity, and a high number of unique alleles existing in these tomato lines. The mean number of alleles per locus was 4.3 and the average poly-morphism information content (PIC) was 0.31. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) clustering at genetic similarity value of 0.85 grouped the inbred lines into four groups, where one USA cultivar formed a separate and more distant cluster. The most similar inbred lines are from USA, both with determinate type, as the most different lines are from USA (Us-16) and Japan (Ja-2) with determinate and indeterminate growth habit, respectively. Clustering was consistent with the known information regarding geographical location and growth habit. The genetic distance information reported in this study might be used by breeders when planning future crosses among these inbred lines.