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目的:研究脑络通对apoE-/-小鼠AS模型血管壁LOX-1、ICAM-1、TNFα的影响。方法:2月龄健康雄性apoE-/-小鼠30只,随机分为模型组、脑络通组、辛伐他汀组,每组10只,高脂饲料饲养10月;同时设立同周龄C57BL/6J小鼠作为正常对照组,正常饲料饲养。10月后,全部小鼠饲料换为正常饲料,同时按照分组,给予相应药物。2月后小鼠取血清,生化法检测血脂;分光光度法检测MDA、SOD、GSH、ox-LDL。取主动脉,HE染色检测病变;western blot检测LOX-1、ICAM-1、TNFα的表达;PCR检测LOX1mRNA的水平。结果:脑络通可以降低ApoE-/-小鼠AS模型血液中TC、TG、LDL-C的水平,调节血脂;升高SOD、GSH的浓度,降低MDA的浓度、降低ox-LDL的浓度,显示其抗氧化作用;降低血管组织ICAM-1与TNFα等炎症因子的表达,降低LOX-1与LOX-1 mRNA的水平;抑制斑块面积。结论:脑络通抑制apoE-/-小鼠AS病变,可能与其降低血脂,抑制氧化应激与炎症因子的表达有关。
Objective: To study the effects of Naoluotong on LOX-1, ICAM-1 and TNFα in apoE-/-mice AS model. METHODS: Twenty healthy male apoE-/- mice aged 2 months were randomly divided into model group, Naoluotong group, and simvastatin group, 10 in each group, and high-fat diet for 10 months. At the same time, the same age C57BL was established. The 6J mouse was used as a normal control group and was fed normally. After 10 months, all mouse feeds were changed to normal feeds, and corresponding drugs were administered according to the grouping. After 2 months, serum was taken from the mice, and blood lipids were measured by biochemical method; MDA, SOD, GSH, and ox-LDL were detected by spectrophotometry. The aorta was removed and lesions were detected by HE staining. Western blot was used to detect the expression of LOX-1, ICAM-1 and TNFα. PCR was used to detect the level of LOX1 mRNA. Results: Naoluotong can reduce the levels of TC, TG, and LDL-C in blood of ApoE-/-mice AS model, regulate blood lipids, increase the concentration of SOD and GSH, decrease the concentration of MDA, and decrease the concentration of ox-LDL. Showed its anti-oxidation; reduce the expression of vascular tissue ICAM-1 and TNFα and other inflammatory factors, reduce the level of LOX-1 and LOX-1 mRNA; inhibition of plaque area. CONCLUSION: Naoluotong inhibits AS lesions in apoE-/- mice, which may be related to lowering blood lipids, inhibiting oxidative stress and expression of inflammatory factors.