论文部分内容阅读
本文研究了盐度胁迫对三疣梭子蟹(Portunustrituberculatus)血淋巴渗透压、鳃丝离子转运酶活力和生物胺含量的影响。结果表明:盐度胁迫(31→26、31→21)对三疣梭子蟹血淋巴渗透压、鳃丝离子转运酶活力和生物胺含量影响显著(P<0.05),而对照组无明显变化。各处理组蟹体渗透压在实验时间内呈峰值变化,至24h时达到最小值,72h后保持稳定,但明显低于对照组水平,且2个处理组无明显差异。各处理组蟹鳃丝Na+-K+-ATPase活力在0~72h内呈峰值变化,至12h达到最大值,72h后恢复至对照组水平并保持稳定;V-ATPase和HCO-3-ATPase活力在实验时间内呈上升趋势,但与对照组差异不显著(P>0.05)。各处理组蟹鳃丝多巴胺(DA)含量在0~24h内呈峰值变化(P<0.05),至12h时达到最大值,24h后恢复至对照组水平并保持稳定;各处理组蟹鳃丝5-羟色胺(5-HT)含量在3~72h内呈峰值变化(P<0.05),至12h时达到最大值,72h后恢复至对照组水平并保持稳定;各处理组蟹鳃丝去甲肾上腺素(NE)含量在0~72h内呈峰值变化,至12h时达到最大值,72h后恢复至对照组水平并保持稳定。由此可见,三疣梭子蟹在低盐胁迫下短时间内达到渗透平衡,而鳃丝Na+-K+-ATPase活力变化稳定滞后,且均表现出明显的时间规律性,同时鳃丝DA和5-HT、NE含量分别与血淋巴渗透压和鳃丝Na+-K+-ATPase活力的变化相吻合,这说明三疣梭子蟹在低盐胁迫下生物胺短时间内发生变化,迅速调节达到渗透平衡,并激活鳃丝Na+-K+-ATPase活力,调节体内外离子浓度维持渗透压的稳定。
In this paper, the effects of salinity on hemolymph osmotic pressure, gill filament ion transport enzyme activity and biogenic amines in Portunustrituberculatus were studied. The results showed that salinity stress (31 → 26,31 → 21) had significant effects on the blood pressure, osmotic pressure, ion transport enzyme activity and biogenic amine content of Portunus trituberculatus (P <0.05), but no significant changes in control group. The osmotic pressure of crabs in each treatment group showed a peak change during the experimental period, reached the minimum value at 24h, remained stable after 72h, but significantly lower than that of the control group, and there was no significant difference between the two treatment groups. The activities of Na + -K + -ATPase in the gill filaments of each treatment group peaked within 0-72h, reaching the maximum at 12h and returned to the level of the control group after 72h and remained stable. The activities of V-ATPase and HCO-3-ATPase in the experiment The time showed an upward trend, but no significant difference with the control group (P> 0.05). The contents of dopamine (DA) in the gill filaments of each treatment group showed a peak value within 0-24 h (P <0.05), reaching the maximum value at 12 h and returned to the control level after 24 h. The gill filaments 5 The content of serotonin (5-HT) peaked at 3-72h (P <0.05), reached its maximum at 12h, returned to the control level after 72h, and remained stable. In each treatment group, the gill filaments of norepinephrine (NE) content peaked within 0 ~ 72h, reached the maximum value at 12h, returned to the control level after 72h and remained stable. Thus, Portunus trituberculatus reached osmotic equilibrium within a short period of time under low salt stress, and the changes of Na + -K + -ATPase activity in gills were stable and lagged, and showed obvious temporal regularity. At the same time, gill filaments DA and 5-HT , Respectively. The results indicated that the bioavailability of bioactive amines in S. portunus changed under the low salt stress in a short period of time, the osmotic balance was rapidly regulated, and the gills Silk Na + -K + -ATPase activity, regulate the concentration of ions in vitro and in vivo to maintain the stability of osmotic pressure.