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目的:建立高分离度快速液相色谱法(RRLC)测定枳实中主要黄酮类成分含量的方法。方法:RRLC色谱条件为Zobax Extend-C18(4.6mm×50mm,1.8μm)色谱柱,流动相为甲醇-2%乙酸(33∶77);检测波长283nm;流速1mL·min-1;柱温30℃。结果:27个批次样品中有19个批次中的主要黄酮类成分为橙皮苷、柚皮苷、新橙皮苷和柚皮芸香苷,按各成分含量比例它们又可以分为两组,一组以柚皮苷和新橙皮苷含量较高,另一组则以柚皮芸香苷和橙皮苷含量较高;其余8个批次的主要黄酮类成分为橙皮苷和柚皮芸香苷,不含有柚皮苷和新橙皮苷,并都以橙皮苷含量较高。各样品主要黄酮成分含量之和为12.4%～38.07%。结论:RRLC方法稳定可靠,分析时间是HPLC的三分之一。枳实中黄酮类成分的种类和各成分的含量比例是不同的。
Objective: To establish a method for the determination of the main flavonoids in Fructus Aurantii Immaturus by high resolution fast liquid chromatography (RRLC). The mobile phase was methanol-2% acetic acid (33:77). The detection wavelength was 283 nm. The flow rate was 1 mL · min-1. The column temperature was 30 ℃. Results: The main flavonoids in 19 batches of 27 batches were hesperidin, naringin, neohesperidin and rindgrass. According to the content of each component, they could be divided into two groups , A group of high content of naringin and neohesperidin, while the other group with rye pomegranate rutin and hesperidin content higher; the remaining eight batches of the main flavonoids hesperidin and pomelo peel Rutin, does not contain naringin and neohesperidin, and higher content of hesperidin. The content of main flavonoids in each sample was 12.4% ~ 38.07%. Conclusion: The RRLC method is stable and reliable, and the analysis time is one-third of HPLC. Fructus aurantii flavonoids in the types of ingredients and the content of the proportion of different.