论文部分内容阅读
目的探讨应用血管形成抑制剂3TSR治疗胰腺癌的疗效。方法在体外培养条件下观察3TSR(1μmol/L)、键择(Gem,1μmol/L)和3TSR+Gem(1μmol/L 3TSR,1μmol/L Gem)对胰腺癌细胞增殖和凋亡的影响。24只免疫缺陷雌鼠胰腺原位接种胰腺癌细胞后,随机分为4组,分别腹腔注射生理盐水(0．2 ml),3TSR(3mg/kg体重)、Gem(150mg/kg体重)和3TSR+Gem(3mg/kg体重3TSR,150mg/kg体重Gem),连续3周后,观察肿瘤体积、肿瘤坏死面积、肿瘤微血管。结果体外培养时3TSR对胰腺癌细胞无增殖抑制及诱导凋亡的作用,3TSR+Gem在体外无协同作用。体内实验时3TSR组、Gem组和3TSR+Gem组肿瘤体积较对照组明显降低；3TSR治疗组肿瘤坏死面积百分比为(53．46±3．23)%,较对照组扩大94．5%；Gem组肿瘤坏死面积百分比明显减小,为(6．75±1．60)%,但3TSR+Gem组肿瘤坏死面积百分比较单纯Gem治疗组明显增加；3个处理组肿瘤平均微血管数分别为31．8±10．9、47．0±17．0、36．9±8．3；微血管面积分别为(289．3±128．7)、(408．7±185．3)、(278．4±150．3)μm~2；微血管密度(3．4±0．9)%、(6．0±1．7)%、(3．7±1．3)%,均显著低于对照组(P＜0．05)。结论3TSR能抑制肿瘤新生血管形成,具有显著减少肿瘤体积、肿瘤血管和增加肿瘤细胞坏死的作用,但与化疗药物Gem合用没有明显提高胰腺癌的治疗效果。
Objective To investigate the curative effect of 3TSR, an inhibitor of angiogenesis, on pancreatic cancer. Methods The effects of 3TSR (1μmol / L), Gem (1μmol / L) and 3TSR + Gem (1μmol / L 3TSR, 1μmol / L Gem) on the proliferation and apoptosis of pancreatic cancer cells were observed under in vitro culture conditions. Twenty-four female immunodeficiency mice were inoculated with pancreatic cancer cells in situ and then randomly divided into 4 groups: intraperitoneal injection of normal saline (0.2 ml), 3TSR (3 mg / kg body weight), Gem (150 mg / kg body weight) and 3TSR + Gem (3TSR at 3mg / kg and Gem at 150mg / kg body weight). After 3 weeks, the tumor volume, tumor necrosis area and tumor microvessel were observed. Results 3TSR in vitro did not inhibit proliferation and induce apoptosis of pancreatic cancer cells. 3TSR + Gem had no synergistic effect in vitro. In vivo, the tumor volume of 3TSR, Gem and 3TSR + Gem groups was significantly lower than that of the control group. The percentage of tumor necrosis area in 3TSR group was (53.46 ± 3.23)%, 94.5% larger than that in control group (6.75 ± 1.60)%, but the percentage of tumor necrosis area in 3TSR + Gem group was significantly higher than that in Gem group. The average number of microvessel in the three treatment groups was 31. 8 ± 10.9,47.0 ± 17.0,36.9 ± 8.3; microvessel area were (289.3 ± 128.7), (408.7 ± 185.3), (278.4 ± 150.3) μm ~ 2; microvessel density (3.4 ± 0.9)%, (6.0 ± 1.7)% and (3.7 ± 1.3)%, respectively, were significantly lower than those in the control group (P <0.05). Conclusions 3TSR can inhibit the formation of neovascularization, which can significantly reduce tumor volume, tumor blood vessels and increase the necrosis of tumor cells. However, combination with chemotherapy drug Gem does not significantly improve the therapeutic effect of pancreatic cancer.