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目的比较体外受精-胚胎移植(IVF-ET)治疗中精卵孵育不同时间对胚胎质量的影响。方法按精卵共孵育时间不同将280个周期采集到的卵子3342枚随机分为短时受精组(A组,精卵共同孵育4～6h后随机选取1/2数量卵子脱颗粒细胞)、长受精组(B组,所剩1/2数量卵子,精卵孵育过夜)。分别比较两组的受精率、多精受精率、卵裂率、优质胚胎率、临床妊娠率和胚胎种植率。结果两组的受精率、多精受精率和卵裂率比较差异无统计学意义(P>0.05);A组的优质胚胎率、临床妊娠率和胚胎种植率显著高于B组(P<0.05)。结论缩短精卵孵育时间不影响受精率及卵裂率,相反能提高胚胎质量,从而提高临床妊娠率。
Objective To compare the effects of different time on the quality of embryos during the in vitro fertilization-embryo transfer (IVF-ET). Methods According to the different time of sperm co-incubation, 3342 ovum collected in 280 cycles were randomly divided into short-term fertilization group (group A, ovariectomized cells randomly selected after 1/2 to 4 hours incubation) Fertilization group (group B, the remaining 1/2 number of eggs, sperm egg incubated overnight). The fertilization rate, fertilization rate, cleavage rate, quality embryo rate, clinical pregnancy rate and embryo implantation rate were compared between the two groups. Results There were no significant differences in fertilization rate, fertilization rate and cleavage rate between the two groups (P> 0.05). The quality embryo rate, clinical pregnancy rate and embryo implantation rate in group A were significantly higher than those in group B (P <0.05 ). Conclusion Shortening the incubation time does not affect the fertilization rate and cleavage rate, on the contrary can improve the quality of embryos, thereby improving the clinical pregnancy rate.