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目的对在广州检出的人类杯状病毒(HuCVs)毒株进行分子生物学鉴定,以确定其是否为新亚型。方法选择检出的HuCVs进行克隆、转化和序列分析,使用PHYLIP进行核酸序列同源性比较,经Treeview1.5绘制进化树。结果206份病毒性腹泻标本检出HuCVs18份,检出率为8.74%(18/206),经序列分析证实均为NVs GⅡ毒株。任选其中6株进行PCR产物的克隆和测序,与GenBank中的参考株比较并绘制遗传进化树,其中HuCV/NVGⅡ003/2003/CHN(32282)、HuCV/NVGⅡ004/2003/CHN(32283)与基因文库中病毒的最高同源性分别为83%和87%。结论广州存在其他国家或地区没有报道过的诺瓦克样病毒新亚型毒株。
OBJECTIVE: To identify molecularly identified human calicivirus (HuCVs) strains in Guangzhou to determine if they are novel subtypes. Methods HuCVs were selected for cloning, transformation and sequence analysis. PHYLIP was used to compare the homology of nucleic acid sequences and the phylogenetic tree was drawn by using Treeview 1.5. Results A total of 18 HuCVs were detected in 206 samples of viral diarrhea. The detection rate was 8.74% (18/206). All the isolates were identified as NVs G Ⅱ by sequence analysis. Six of them were selected for cloning and sequencing of PCR products, compared with the reference strains in GenBank and the phylogenetic tree was drawn. HuCV / NVGⅡ003 / 2003 / CHN (32282), HuCV / NVGⅡ004 / 2003 / CHN The highest homology of the virus in the library was 83% and 87%, respectively. Conclusion There are no new subtypes of Norwalk-like virus that have not been reported in other countries or regions in Guangzhou.