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目的观察铅、硒对大鼠海马突触结构参数的影响,探讨铅致学习记忆损害机制及硒的拮抗作用。方法 Wistar大鼠随机分为对照组、单独染铅组、单独加硒组和铅硒同时组,至观察终点测定血铅和血硒值,利用透射电镜测定突触结构参数。结果单独染铅组较对照组,血铅值显著增高,突触间隙宽度明显增大,突触后致密物厚度、活性带长度及界面曲率明显减小(P<0.05);铅硒同时组与单独染铅组相比,血铅值降低,突触间隙宽度显著减小,突触后致密物厚度及活性带长度明显增大(P<0.05)。结论铅暴露可改变大鼠海马突触结构参数,从而对学习记忆造成影响;硒对铅致大鼠海马突触结构改变具有保护作用。
Objective To observe the effect of lead and selenium on synaptic structure parameters of hippocampus in rats and explore the mechanism of lead-induced learning and memory impairment and the antagonism of selenium. Methods Wistar rats were randomly divided into control group, lead group alone, selenium alone group and lead and selenium group. Blood lead and blood selenium were measured at the end of observation. Synaptic structural parameters were determined by transmission electron microscopy. Results Compared with the control group, the levels of lead in blood lead significantly increased and the width of synaptic cleft significantly increased, the thickness of synaptic dense, length of active zone and curvature of interface decreased significantly (P <0.05) The blood lead decreased, the width of the synaptic cleft significantly decreased, and the thickness of the postsynaptic density and the length of the active band increased significantly (P <0.05). Conclusion Lead exposure can change the synaptic structure parameters in hippocampus of rats, which may affect learning and memory. Selenium has a protective effect on the synaptic structure of hippocampus in lead-induced rats.