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目的观察肺炎支原体感染对BALB/c小鼠气道IFN-γ和IL-4、IL-5、IL-10以及气道阻力的影响。方法建立肺炎支原体经呼吸道感染的BALB/c小鼠模型,应用RT-PCR和HE染色的方法进行模型的鉴定,用ELLSA的方法检测BALF中细胞因子的含量,AniRes2005动物肺功能仪测各组小鼠肺的吸气阻力、呼气阻力、顺应性。结果 MP感染BALB/c小鼠后IFN-γ和IL-4、IL-5、IL-10细胞因子较正常对照组明显升高(P<0.05);吸气阻力、呼气阻力较正常对照组增加(P<0.05),顺应性降低较正常对照组增加(P<0.05)。结论 MP肺炎感染BALB/c小鼠后气道阻力增加、顺应性降低,而且气道中的炎性因子持续存在。
Objective To investigate the effects of Mycoplasma pneumoniae infection on airway IFN-γ, IL-4, IL-5, IL-10 and airway resistance in BALB / c mice. Methods BALB / c mice model with Mycoplasma pneumoniae infection was established. The model was identified by RT-PCR and HE staining. The levels of cytokines in BALF were detected by ELLSA. Rat lung suction resistance, expiratory resistance, compliance. Results The cytokines of IFN-γ, IL-4, IL-5 and IL-10 in MP infected BALB / c mice were significantly higher than those in control group (P <0.05). The inspiratory resistance and expiratory resistance were significantly higher than those in normal control (P <0.05), the compliance decreased compared with the normal control group (P <0.05). Conclusion MP pneumonia in BALB / c mice increased airway resistance, decreased compliance, and persistent inflammatory cells in the airway.