论文部分内容阅读
不同剂量即~(60)Co γ线、中子及X射线照射正常人血后,以丝裂原PHA、ConA、LPS及PWM诱导血内淋巴细胞体外培养转化实验,研究淋巴细胞亚群的辐射损伤效应。实验用~3H-TdR、~(14)C-UR及~(14)C-缬氨酸放射性核素标记化合物示踪技术,观察不同丝裂原诱导人血淋巴细胞的增殖分化动态;电离辐射对细胞内DNA、RNA及蛋白质合成的抑制作用,并比较其辐射损伤效应。结果:(1)不同丝裂原诱导的正常人血淋巴细胞增殖分化特性各异,分属不同的淋巴细胞亚群。(2)实验以DNA及蛋白质合成中,放射性同位素参入CPM值为指标,观察到ConA、PHA诱导的T淋巴细胞亚群,其辐射损伤效应大于LPS、PWM诱导的B淋巴细胞。(3)淋巴细胞受中子照射后的损伤效应大于γ射线及X射线照射。(4)不同丝裂原诱导的正常人血麻巴细胞亚群,受照射后其细胞DNA链断裂修复能力愈强,该细胞的辐射敏感性就愈低。
Different concentrations of ~ (60) Co γ-ray, neutron and X-ray irradiation of normal human blood, with mitogen PHA, ConA, LPS and PWM-induced transfection of blood lymphocytes in vitro experiments to study the lymphocyte subsets of radiation Damage effect. The 3H-TdR, ~ (14) C-UR and ~ (14) C-valine radionuclide labeling compounds were used to observe the proliferation and differentiation of human lymphocytes induced by different mitogen. Ionizing radiation Inhibit the synthesis of DNA, RNA and protein in cells, and compare their radiation damage effects. Results: (1) The proliferation and differentiation of normal human lymphocytes induced by different mitogen origins were different and belong to different lymphocyte subsets. (2) In the experiment, the DNA and protein synthesis, radioactive isotope incorporation CPM value as an indicator, ConA, PHA-induced T lymphocyte subsets, the radiation damage effect than LPS, PWM-induced B lymphocytes. (3) The damage effect of lymphocytes irradiated by neutron is greater than γ-ray and X-ray irradiation. (4) The normal human hematopoietic stem cell subpopulation induced by different mitogen was stronger, and the stronger the DNA strand break repair ability was, the lower the radiosensitivity of the cells was.