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以苯甲酸为内标，建立了ＨＰＬＣ测定动物血浆、尿、粪及心、肝、脾、肺、肾等组织匀浆中淫羊藿苷的浓度．色谱柱采用ＺｏｒｂａｘＯＤＳ，流动相为四氢呋喃—水—冰醋酸（２０７５５），紫外检测波长２７０ｎｍ．生物样品经乙酸乙酯提取处理后可获得良好分离，血药浓度在１～１２８μｇ／ｍＬ范围内呈线性关系，相关系数０．９９９９，最低检测浓度为０．５μｇ／ｍＬ．生物样品的平均方法回收率９５．２６％～１０１．３％，平均提取回收率７３．５７％～９７．１０％，日内、日间精密度ＲＳＤ２．０％～７．１％．
Using benzoic acid as internal standard, HPLC was established for determination of icariin in plasma, urine, feces and heart, liver, spleen, lung, kidney and other tissue homogenates. ZorbaxODS was used as the chromatographic column. The mobile phase was tetrahydrofuran-water-glacial acetic acid (20755). The UV detection wavelength was 270nm. The biological samples can be well separated after being extracted by ethyl acetate. The linear range of the concentration is 1-18μg / mL, the correlation coefficient is 0.9999, and the lowest detection concentration is 0.5μg / mL. The average recovery rate of biological samples was 95.26% ~ 101.3% with the average recovery of 73.57% ~ 97.10%. The intra-day and inter-day precision RSD2.0% ~ 7.1%.