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目的:探讨消瘀片减少粥样硬化血管壁中平滑肌细胞数的作用机制。方法:雄性新西兰兔高脂饮食加腹主动脉剥脱术制成腹主动脉粥样硬化模型,通过显微镜检查、放免法、比色法、原位末端标记及图象分析技术分别测定连续给予消瘀片0.16-0.32g·kg~(-1)·d~(-1)治疗16周后血管壁组织的形态学、内皮素(ET)-l、一氧化氮(NO)含量及细胞凋亡的变化。结果:消瘀片治疗16周后,粥样硬化血管壁的内膜厚度和平滑肌细胞数明显减少,血管壁组织中的ET-l含量降低8.2％—42.6％,NO含量增加7.5％-54.2％,凋亡细胞阳性反应颗粒明显减少,其所占的面积和积分光密度值在粥样硬化组是(846±308)μm~2和3425±1374,消瘀片0.32g/kg组是(225±60)μm~2和1445±606。结论:消瘀片通过降低血管壁中的ET-l抑制平滑肌细胞的增生,通过增加血管壁中的NO诱导细胞凋亡。
Objective: To investigate the mechanism of Xiaoyu tablets in reducing the number of smooth muscle cells in atherosclerotic vessel wall. Methods: Atherosclerosis models of abdominal aorta were made in male New Zealand rabbits with high-fat diet and abdominal aorta stripping. Microscopic examination, radioimmunoassay, colorimetry, end-site labeling and image analysis were used to determine the effects of continuous administration The morphological changes of vascular wall, endothelin (ET) -1, nitric oxide (NO) content and apoptosis after 16 weeks treatment of 0.16-0.32g · kg -1 (-1) d -1 Variety. Results: After 16 weeks of treatment, the intima thickness and the number of smooth muscle cells in atherosclerotic vessel wall decreased significantly, the content of ET-1 in vessel wall tissue decreased by 8.2% -42.6% and the content of NO increased by 7.5% -54.2% (846 ± 308) μm ~ 2 and 3425 ± 1374 in atherosclerosis and 0.32g / kg in Xiaoyu tablets were (225 ± 60) μm ~ 2 and 1445 ± 606. Conclusion: Xiaoyu Tablet can inhibit the proliferation of smooth muscle cells by decreasing ET-1 in the vascular wall and induce apoptosis by increasing NO in the vascular wall.