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目的 深入了解UL2基因与HSV 1在三叉神经节潜伏感染的相关性。方法 选择HSV 1国际参考标准株F株、HSV 1RE(株 ) (UL2基因插入突变株 )和其亲代HSV 1△ 30 5株 (TK- )三株病毒进行对比分析研究。对三株HSV 1病毒腹腔免疫接种小鼠双侧三叉神经节分别进行PCR扩增。结果 HSV 1 (RE)和HSV 1 (△ 30 5)组HSV 1潜伏感染率明显低于HSV 1 (F)组 (P <0 0 2 5) ,提示UL2基因可能在HSV 1形成潜伏感染过程中起一定作用。经HSV 1 (F)病毒攻击实验后三个实验组小鼠 ,其潜伏感染率无明显变化 ,而对照组小鼠潜伏感染率明显高于HSV 1 (RE)组和HSV 1 (△ 30 5)组 (P <0 0 1 )。结论 HSV 1 (RE)株的免疫接种可以预防HSV 1潜伏感染的建立。同时也为HSV 1RE株作为神经系统疾病基因治疗载体提供了理论依据
Objective To understand the relationship between UL2 gene and HSV 1 latent infection in the trigeminal ganglion. Methods HSV 1 international reference strain F strain, HSV 1RE strain (UL2 gene insert mutant strain) and its parental HSV 1 △ 30 5 strain (TK-) were selected for comparative study. Three strains of HSV 1 were intraperitoneal immunized mice with bilateral trigeminal ganglion for PCR amplification. Results The latent infection rate of HSV 1 in HSV 1 (RE) and HSV 1 (△ 30 5) group was significantly lower than that in HSV 1 (F) group (P <0 0 2 5), suggesting that UL2 gene might be involved in latent infection during HSV 1 formation Play a role. There was no significant change in the latent infection rate in mice in the three experimental groups after challenge with HSV 1 (F) virus, while the latent infection rate in control mice was significantly higher than that in HSV 1 (RE) group and HSV 1 (△ 30 5) Group (P <0 0 1). Conclusion The immunization with HSV 1 (RE) strain can prevent the latent infection of HSV 1. It also provides a theoretical basis for HSV 1RE strain as gene therapy vector for neurological diseases