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目的探寻复方蛇龙胶囊的有效物质基础。方法将MPC5细胞分为空白对照组,模型组,白花蛇舌草多糖系列浓度组,穿山龙总皂苷系列浓度组,鬼箭羽总黄酮系列浓度组,白花蛇舌草多糖加穿山龙总皂苷系列浓度组,白花蛇舌草多糖加鬼箭羽总黄酮系列浓度组,穿山龙总皂苷加鬼箭羽总黄酮系列浓度组,复方蛇龙全方系列浓度组及单味穿山龙总皂苷与全方系列浓度对比组。采用CCK-8检测细胞活力来探寻发挥药效的物质基础。结果与空白对照组相比,模型组细胞活力明显下降(P<0.01);与模型组相比,单味穿山龙总皂苷系列浓度组,含有1μg/ml的白花蛇舌草总多糖和穿山龙总皂苷组及含有1μg/ml的穿山龙总皂苷组和鬼箭羽总黄酮组以及含全方100ng/ml组细胞活力增加(P<0.05),其他各组与模型组未见明显差异(P>0.05)。结论复方蛇龙胶囊对MPC5确有保护作用,且单味穿山龙总皂苷与全方疗效相当。
Objective To explore the effective material base of compound snake dragon capsule. Methods MPC5 cells were divided into blank control group, model group, Hedyotis diffusa polysaccharide concentration group, Pangasius total saponin concentration group, ghost arrow feather total flavonoid series concentration group, Hedyotis diffusa Willd. , Hedyotis diffusa Willd polysaccharides plus Ghost Arrows total flavonoids concentration group, the total Saponins Ghost Arrows total flavonoids concentration group, the compound Serpentine all series of concentration group and a single Saponins total saponins with the whole series of concentration control group . CCK-8 was used to detect cell viability to explore the material basis for efficacy. Results Compared with the blank control group, the viability of the model group was significantly decreased (P <0.01). Compared with the model group, the total concentration of polysaccharides in the total concentration of 1 g / ml Hedyotis diffusa, (P <0.05). There was no significant difference between the other groups and the model group (P> 0.05) . Conclusion Compound Serpentine Capsule has a protective effect on MPC5, and the single herb of Salviae Miltiorrhiza has the same curative effect as the whole prescription.