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本文介绍了利用生物发光技术测定靶细胞释放出的ATP浓度来计算NK细胞活性的方法。实验中发现效应细胞在培养过程中细胞内ATP自然释放现象;培养后细胞内ATP浓度(3.4±0.21)比培养前低(4.7±0.3),P<0.05。对细胞内ATP提取方法做简要探讨,以细胞冻融法较理想。同时对25名中、晚期肿瘤病人和50名献血员NK细胞活性进行了测定,结果肿瘤病人NK活性(11.7±4.5)明显低于对照组(26.5±3.0),P<0.01。该法测定NK活性较为简便并避免了同位素半衰期短和放射性污染等缺点。
This article describes the use of bioluminescence technology to determine the concentration of ATP released by target cells to calculate NK cell activity. The natural release of intracellular ATP was observed in effector cells during the experiment. The intracellular ATP concentration (3.4 ± 0.21) was lower than that before culture (4.7 ± 0.3), P <0.05. A brief discussion of intracellular ATP extraction methods, cell freezing and thawing method is more ideal. At the same time, NK cell activity of 25 patients with advanced cancer and 50 blood donors was measured. The results showed that the activity of NK in tumor patients (11.7 ± 4.5) was significantly lower than that in control group (26.5 ± 3.0), P <0.01. The assay of NK activity is simpler and avoids the disadvantages of short isotope half-life and radioactive contamination.