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目的探讨卵巢子宫内膜异位囊肿及卵巢子宫内膜样癌组织中抑癌基因PTEN蛋白表达及意义。方法利用免疫组化SP法和蛋白质免疫印迹法检测1985-2005年中国医科大学附属第一医院妇科手术切除的卵巢子宫内膜异位囊肿30例、卵巢子宫内膜样癌标本30例和正常增生期子宫内膜20例组织中PTEN蛋白的表达。结果PTEN蛋白主要定位于细胞浆中,在正常子宫内膜均为阳性表达,在卵巢子宫内膜异位囊肿组织中染色强度和范围较正常子宫内膜弱(0.77±0.08对1.59±0.12,P<0.05);而在卵巢子宫内膜样癌组织中表达明显下降,与正常子宫内膜和卵巢子宫内膜异位囊肿比较,差异有统计学意义(分别为0.55±0.08对1.59±0.12,0.55±0.08对0.77±0.08,P<0.05),且与组织分化程度、临床病理分期及有无子宫内膜异位症病史有关;分期越晚,分化程度越低,蛋白表达越少。结论PTEN蛋白失活可能是卵巢子宫内膜异位囊肿向卵巢子宫内膜样癌发生过程中的重要事件,且检测PTEN蛋白表达对判断患者预后具有一定意义。
Objective To investigate the expression of tumor suppressor gene PTEN in ovarian endometriotic cyst and ovarian endometrioid carcinoma and its significance. Methods Immunohistochemistry (SP) and Western blotting were used to detect 30 cases of ovarian endometriotic cyst excised from the first affiliated hospital of China Medical University from 1985 to 2005, 30 cases of ovarian endometrioid carcinoma and normal hyperplasia Expression of PTEN protein in 20 cases of endometrium. Results PTEN protein mainly localized in the cytoplasm and expressed in normal endometrium. The intensity and extent of staining in endometriotic ovarian tissue were weaker (0.77 ± 0.08 vs. 1.59 ± 0.12, P <0.05). However, the expression was significantly decreased in endometrioid ovarian cancer tissues. Compared with normal endometrium and ovarian endometriotic cyst, the difference was statistically significant (0.55 ± 0.08 vs. 1.59 ± 0.12, 0.55 ± 0.08 vs 0.77 ± 0.08, P <0.05), and related to the degree of histological differentiation, clinical pathological stage and the presence or absence of endometriosis; the later the stage, the lower the degree of differentiation, the less protein expression. Conclusion Inactivation of PTEN protein may be an important event in the process of ovarian endometriosis into ovarian endometrial carcinomas. Detecting the expression of PTEN protein is of great significance in judging the prognosis of patients.