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目的:研究复方地黄对6-羟基多巴胺(6-hydroxydopamine,6-OHDA)诱导的帕金森病(Parkinson’s disease,PD)大鼠多巴胺能神经元的保护作用及其可能机制。方法:40只SD雄性大鼠,分为正常组、假手术组、模型组、模型组+复方地黄组(即干预组)。观察PD大鼠药物诱发的旋转行为;免疫组化法观察黑质酪氨酸羟化酶(tyrosine hydroxylase,TH)阳性神经元数目及胶质细胞源性神经营养因子(glial cell line-derivedneurotrophic factor,GDNF)表达水平的变化。结果:干预组与模型组间的旋转圈数有明显差异(P<0.05);模型组黑质TH阳性神经元数目(52.9±2.2)明显少于正常组(160.3±2.4)、假手术组(161.6±3.4)及干预组(61.5±2.6)(P<0.05);在大鼠黑质和纹状体部位,干预组GDNF阳性细胞数量比正常组、假手术组、模型组均明显增加(P<0.05)。结论:复方地黄方能明显改善PD大鼠的旋转行为,拮抗6-羟基多巴胺诱导的PD大鼠黑质多巴胺能神经元的丢失,其保护作用可能与促进内源性GDNF分泌增加有关。
Objective: To study the protective effect of compound Rehmanniae on dopaminergic neurons induced by 6-hydroxydopamine (6-OHDA) in Parkinson’s disease (PD) rats and its possible mechanism. Methods: Forty SD male rats were divided into normal group, sham operation group, model group, model group and compound rhubarb group (intervention group). The drug-induced rotational behavior of PD rats was observed. The number of tyrosine hydroxylase (TH) positive neurons and glial cell line-derived neurotrophic factor GDNF) expression level changes. Results: The numbers of TH positive neurons in substantia nigra (52.9 ± 2.2) in the model group were significantly less than those in the normal group (160.3 ± 2.4), while those in the sham group 161.6 ± 3.4) and the intervention group (61.5 ± 2.6) (P <0.05). In the substantia nigra and striatum, the number of GDNF positive cells in the intervention group was significantly higher than that in the normal group, the sham operation group and the model group (P <0.05). CONCLUSION: Fufang Dihuang Decoction can significantly improve the rotation behavior of PD rats and antagonize the loss of dopaminergic neurons of substantia nigra induced by 6-hydroxydopamine in PD rats. The protective effect may be related to the increase of secretion of endogenous GDNF.