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目的 :探讨富于T细胞 /组织细胞B细胞淋巴瘤 (TCRBCL)的诊断。方法 :用S P石蜡免疫组化法检测 2 2例依据形态学诊断的霍奇金淋巴瘤的瘤细胞和背景细胞的免疫表型。结果 :4/ 2 2例是TCRBCL ,3例富于T小淋巴细胞 ,1例富含组织细胞 ;瘤细胞 3例呈中心母细胞样和免疫母细胞样 ,1例呈腔隙型细胞样 ,弥漫散在分布。免疫组化瘤细胞呈CD2 0 (+ )、CD15(- )、CD30 (- )、CD2 1(- )、vimentin(- )。背景细胞CD45RO(+ ) /CD6 8(+ )细胞占绝对优势 ,为浸润细胞的 70 %～ 90 % ;CD2 0 (+ )细胞散在 ,CD5 7(+ )稀少。 16例为经典型霍奇金淋巴瘤 (CHL) ,瘤细胞为CD15 (+ ) (75 % )、CD30 (+ ) (10 0 % )、vi mentin(+ ) (19% )、CD2 1(- )、CD2 0 (- )及CD45 (- ) ,背景细胞CD45RO(+ )和CD2 0 (+ )数量基本相等 ,CD5 7(+ )较少。 1例为结节性淋巴细胞为主型霍奇金淋巴瘤 (NLPHL) ,瘤细胞呈CD2 0 (+ )、CD45 (+ )、CD30 (- )、CD15 (- ) ,而背景细胞中CD5 7(+ )较多。结论 :石蜡免疫组化在TCRBCL诊断中起重要作用 ,而且也应用于CHL、NLPHL及TCRBCL间鉴别诊断。
Objective: To investigate the diagnosis of T-cell/tissue-cell B-cell lymphoma (TCRBCL). METHODS: The immunophenotypes of tumor cells and background cells in 22 cases of Hodgkin’s lymphoma diagnosed according to morphology were detected by immunohistochemical method with S P paraffin. Results: 4/22 cases were TCRBCL, 3 cases were rich in T-lymphocytes, 1 case was rich in histiocytes; 3 cases of tumor cells were centroblast-like and immunoblast-like, 1 case was lacunar-like, Diffuse diffuse distribution. The immunohistochemical tumor cells showed CD20 (+), CD15 (-), CD30 (-), CD21 (-), and vimentin (-). The background cells CD45RO(+)/CD68(+) cells predominate, accounting for 70% to 90% of infiltrating cells; CD2 0(+) cells are scattered and CD5 7(+) are rare. 16 cases were classical Hodgkin’s lymphoma (CHL), tumor cells were CD15 (+) (75%), CD30 (+) (100%), vi mentin (+) (19%), CD2 1 (- ), CD2 0 (-) and CD45 (-), background cells CD45RO (+) and CD2 0 (+) are essentially equal, CD5 7 (+) less. One case was nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL). The tumor cells were CD20 (+), CD45 (+), CD30 (-), CD15 (-), while the background cells were CD5 7 (+) more. Conclusion: Paraffin immunohistochemistry plays an important role in the diagnosis of TCRBCL, and it is also used in the differential diagnosis of CHL, NLPHL and TCRBCL.