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目的:探讨人参炔醇对氧糖剥夺(OGD)致神经细胞损伤的保护作用。方法:体外培养PC12细胞,分为正常对照组、OGD组、人参炔醇组、尼莫地平组,加入含0.5 mmol.L-1 Na2SO4的无糖Earle’s液建立OGD损伤模型,以人参炔醇(10,20mg.L-1)、尼莫地平(100μmol.L-1)处理。采用荧光显微镜观察细胞形态,Hoechst和PI双染流式细胞分析仪检测凋亡率,测定培养上清液中乳酸脱氢酶(LDH)的活性与细胞Ca2+浓度,观察人参炔醇对OGD致PC12细胞损伤的保护作用。结果:人参炔醇能够减少OGD损伤PC12细胞LDH的释放(P<0.05,P<0.01),抑制Ca2+的过量产生(P<0.05,P<0.01)、显著减少细胞凋亡及坏死(P<0.05,P<0.01)。结论:人参炔醇对OGD致神经细胞损伤具有保护作用。
Objective: To investigate the protective effect of ginsenositol on neuronal damage induced by oxygen glucose deprivation (OGD). Methods: PC12 cells were cultured in vitro and divided into normal control group, OGD group, panaxynol group and nimodipine group. OGD injury model was established by adding sugar-free Earle’s solution containing 0.5 mmol.L-1 Na2SO4, 10, 20 mg.L-1), nimodipine (100 μmol.L-1). The cell morphology was observed by fluorescence microscopy. The apoptosis rate was detected by Hoechst and PI double-flow cytometry. The activity of lactate dehydrogenase (LDH) and the concentration of Ca2 + in the culture supernatant were measured. The effect of ginsenositol on OGD induced PC12 Cell injury protection. Results: Panaxadiol could reduce the release of LDH (P <0.05, P <0.01), inhibit the overproduction of Ca2 + (P <0.05, P <0.01), and significantly reduce the apoptosis and necrosis of PC12 cells , P <0.01). CONCLUSION: Ginsenosyl alcohol can protect OGD-induced neuronal injury.