云贵地区有丰富的中草药资源,1975年起我们对民间验方或采集标本进行了抗病毒作用筛选。一、组织培养采用原代人胚肾细胞;病毒用流感病毒A_3/昆军—75王株、A_3/昆军—75付株,鼻病毒17型,腺病毒7型;药物用水煎剂,测定药液对细胞之无毒反应界限后,进行病毒抑制试验,一般均经重复。方法为先加入药液,次日换液,加入同量含药液之维持液,并加入0.1毫升含100 The Yunnan-Guizhou region is rich in Chinese herbal medicine resources. Since 1975, we have conducted antiviral screening of non-governmental prescriptions or collected specimens. First, tissue culture uses primary human embryonic kidney cells; virus influenza virus A_3/Kunjun-75 Wang strain, A_3/Kunjun-75 strain, rhinovirus type 17 and adenovirus type 7; drug decoction, determination After the limits of the drug’s nontoxic response to the cells, virus inhibition tests were performed and were generally repeated. The method is to add the liquid first, change the liquid the next day, add the same amount of liquid containing the liquid, and add 0.1 ml containing 100