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本文研究吗啉(M)、4,4’-二硫双吗啉(DTDM)、吗啉基巯基苯并噻唑(MMBT)及2-巯基苯并噻唑(MBT)致敏反应的生物化学基础。选用半胱氨酸(Cys)、赖氨酸(Lys)、甘氨酸(Gly)分别与上述4种化学物质相加进行结合反应。结果表明除M外,其他各组均有新的结合物形成,这与动物实验结果一致。DTDM致敏可能是由于二硫键与氨基酸的—SH及—NH_2结合;MMBT与氨基酸的结合可能通过相似反应机理。MBT的—SH借氧化及(或)硫酯形成与氨基酸的—COOH结合,后者所形成的加成物可能是导致MBT致敏的主要致敏原。这种体外试验可用来预测一些化学物质的致敏潜力。
In this paper, the biochemical basis of sensitization of morpholine (M), 4,4’-dithioborpholine (DTDM), morpholinylmercaptobenzothiazole (MMBT) and 2-mercaptobenzothiazole (MBT) was studied. Cysteine (Cys), lysine (Lys), glycine (Gly) were selected for the binding reaction with the above four chemicals respectively. The results showed that in addition to M, the other groups have a new combination of formation, which is consistent with the results of animal experiments. DTDM sensitization may be due to the disulfide bond with the -SH and -NH_2 of amino acids; the binding of MMBT to amino acids may be through a similar reaction mechanism. The -SH of MBT forms an -COOH bond with an amino acid by oxidation and / or thioester, which forms an adduct that may be the major allergen causing MBT sensitization. This in vitro test can be used to predict the sensitization potential of some chemicals.