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有球根海棠组织培养中发现，在较高温度（28℃左右）下几乎所有外植体直接出芽，但生长缓慢；在较低温度（22℃左右）下绝大多数经愈伤组织出芽。因此，在较高温度诱导出芽后降低培养温度可提高组培效率。
In the tissue culture of Begonia tuberculus, almost all the explants germinated directly at higher temperature (about 28 ℃), but they grew slowly. Most of the explants emerged callus at lower temperature (about 22 ℃). Therefore, inducing budding at a higher temperature to reduce the culture temperature can improve tissue culture efficiency.