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目的探讨肝性脑病(HE)大鼠肝脏及脑组织中的蛋白质硝基化和细胞凋亡与HE发病的关系。方法雄性Wistar大鼠随机分为HE组和空白对照组。HE组用硫代乙酰胺(TAA)制造大鼠HE模型。采用链霉亲和素-生物素-酶复合物(SABC)免疫组化法检测肝脏和脑组织硝基化蛋白质和一氧化氮合酶(NO S);用原位末端脱氧核糖核酸转移酶标记法(TUNEL)检测细胞凋亡,并计算凋亡指数;空白对照组大鼠用生理盐水做平行对照。结果 HE组大鼠肝脏和脑组织中检测到明显的硝基化蛋白质沉积,而对照组少见;HE组大鼠肝脏和脑组织中检测到NO S的表达,对照组甚少;HE组大鼠肝脏和脑组织细胞凋亡指数较对照组明显增高(P<0.01)。结论 HE与其肝脏和脑组织蛋白质硝基化关系密切。
Objective To investigate the relationship between protein nitration and apoptosis in hepatic and brain tissues of rats with hepatic encephalopathy (HE) and the pathogenesis of HE. Methods Male Wistar rats were randomly divided into HE group and blank control group. The rat HE model was made by thioacetamide (TAA) in HE group. The nitrotyrosine protein and nitric oxide synthase (NO S) in liver and brain tissue were detected by streptavidin-biotin-enzyme complex (SABC) immunohistochemistry. The in situ terminal deoxyribonucleic acid transferase TUNEL method was used to detect apoptosis and calculate apoptotic index. Blank control group rats were treated with saline as parallel control. Results Significant nitrosylation protein deposition was found in the liver and brain tissue of HE group, while the control group was rare. The expression of NO S was detected in liver and brain tissue of HE group, The apoptosis index of liver and brain tissue was significantly higher than that of the control group (P <0.01). Conclusion HE is closely related to the protein nitration of liver and brain tissue.