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目的 寻找新的制备神经生长因子的材料和方法。方法 利用牛精液为原料，进行两次阳离子交换层析。结果 获得了纯的牛神经生长因子，相对分子质量为30000，比活性为400000BU/mg，FPLC检测结果为一个峰，电泳检测纯度大于95％。结论 可以从牛精液中制备神经生长因子。
Objective To search for new materials and methods for preparation of nerve growth factor. Methods Bovine semen was used as raw material for twice cation exchange chromatography. Results Pure bovine nerve growth factor was obtained with a molecular weight of 30,000 and a specific activity of 400000BU / mg. The results of FPLC showed a peak and the purity of electrophoresis was more than 95%. Conclusion Nerve growth factor can be prepared from bovine sperm.