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采用富集筛选法,以精氨酸为唯一氮源从羊草根际土壤中筛选出具有产多胺活力的菌株ccx5,探索产多胺细菌ccx5对植物生长的作用机制。以黄瓜为材料,采用营养液沙培的方法,研究了产多胺细菌ccx5对NaCl胁迫下的黄瓜幼苗生长的影响。结果表明:在NaCl的胁迫下,黄瓜幼苗的生长明显受到了抑制,且叶片中的叶绿素含量减少、丙二醛含量升高,抗氧化酶活性显著提高。而接种了产多胺菌ccx5的试验组的黄瓜幼苗的生长抑制得到缓解,叶片中的叶绿素含量和抗氧化酶活性明显高于NaCl处理组,但丙二醛含量有所降低。试验表明在NaCl胁迫下,产多胺细菌ccx5影响了植物的代谢过程,提高了黄瓜幼苗对盐胁迫的抗性。
Using enrichment screening method, arginine is the only nitrogen source, the strain ccx5 producing polyamine activity was screened from the rhizosphere soil of Leymus chinensis to explore the mechanism of action of polyamines bacterial ccx5 on plant growth. Using cucumber as material, the effect of producing polyamine amine bacteria ccx5 on the growth of cucumber seedling under NaCl stress was studied by using nutrient sap culture method. The results showed that under the NaCl stress, the growth of cucumber seedlings was significantly inhibited, and the content of chlorophyll decreased, the content of malondialdehyde increased and the activity of antioxidant enzymes increased significantly. However, the growth inhibition of cucumber seedlings inoculated with polyamycetic bacteria ccx5 was relieved. The chlorophyll content and antioxidant enzyme activity in leaves were significantly higher than those in NaCl treatment group, but the content of malondialdehyde decreased. The results showed that under the NaCl stress, the production of polyamine bacteria ccx5 affected plant metabolism and increased the resistance of cucumber seedlings to salt stress.