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采用L-半胱氨酸、巯基乙酸为稳定剂水相合成Cd Te量子点,且以Cd Te量子点为能量供体、孔雀石绿为能量受体建立荧光共振能量转移体系,从而实现孔雀石绿含量的同步荧光猝灭法测定。结果表明,当固定波长差为220 nm时,Cd Te量子点的同步荧光最大发射波长位于323 nm。在最优实验条件下,体系的同步荧光淬灭强度与孔雀石绿的浓度在0.5~10.0μmol/L范围内呈良好的线性关系,相关系数为0.9995,方法的检出限为10 nmol/L。该方法用于实际水样中孔雀石绿含量的测定,加标回收率为94%~105%。
CdTe quantum dots were synthesized by L-cysteine and thioglycolic acid as stabilizers, and the fluorescence resonance energy transfer system was established by using CdTe quantum dots as energy donor and malachite green as energy acceptor, Determination of Green Content by Synchronous Fluorescence Quenching. The results show that when the fixed wavelength difference is 220 nm, the maximum fluorescence emission wavelength of CdTe quantum dots is at 323 nm. Under the optimal experimental conditions, the synergistic fluorescence quenching intensity of the system showed a good linear relationship with the concentration of malachite green in the range of 0.5 ~ 10.0μmol / L with the correlation coefficient of 0.9995. The detection limit of the method was 10 nmol / L . The method was applied to the determination of the malachite green content in the actual water sample with the recoveries of 94% -105%.