在饲料中添加羰基铁喂养大鼠 ,形成肝脏内铁含量不同的铁过载动物模型 ,体外研究肝组织内不同的铁含量对乙醇诱导肝组织氧化损伤的影响 ,探讨乙醇和铁在诱导肝组织损伤过程中的交互作用。结果显示 :饲料中添加铁剂可明显增加铁在肝脏内的蓄积 ,肝脏内铁含量随喂养时间而增加 ,与对照组比较差异有非常显著意义 (P <0 0 1)。肝脏内不同的铁含量均可明显增加乙醇诱导的肝组织脂质过氧化反应。分别与单纯乙醇和相应的单纯铁过载组比较 ,铁过载组肝组织匀浆中加入一定剂量的乙醇 ,肝组织中MDA含量增加 ,GSH含量减少 ,差异有显著性。肝组织内铁含量与MDA成正相关 ,与GSH含量成负相关 ,且均有显著性差异 (P <0 0 5 )。存在剂量 效应关系。提示 :乙醇诱导的肝组织脂质过氧化反应与肝脏内铁的状况关系密切 ,铁可明显增加乙醇诱导的肝组织脂质过氧化反应。铁和乙醇在诱导肝组织脂质过氧化损伤过程中显示有效交互作用。 Iron carbonyl diet was used to feed rats to form iron overload animal models with different iron contents in liver. The effects of different iron contents in liver tissue on oxidative damage induced by ethanol were studied in vitro. The effects of ethanol and iron on liver injury induced Interactions in the process. The results showed that the addition of iron to the diet significantly increased the accumulation of iron in the liver, and the content of iron in the liver increased with the feeding time, which was significantly different from the control group (P <0.01). Different iron content in the liver can significantly increase ethanol-induced lipid peroxidation in liver tissue. Compared with pure ethanol and the corresponding simple iron overload group respectively, a certain dose of ethanol was added into liver homogenate of iron overload group. The content of MDA and the content of GSH in liver tissue were increased, the difference was significant. The content of iron in liver tissue was positively correlated with MDA and negatively correlated with GSH content (P <0.05). There is a dose-response relationship. Tip: ethanol-induced liver lipid peroxidation and liver iron status closely related to iron can significantly increase ethanol-induced liver lipid peroxidation. Iron and ethanol showed an effective interaction in inducing lipid peroxidation injury in liver tissues.