论文部分内容阅读
目的运用蛋白激酶C(PKC)特异性抑制剂Bis-1,初步探讨细胞内PKC信号途径在A23187诱导HL-60细胞向树突状细胞(dendritic cell,DC)分化过程中的作用,了解细胞内钙离子信号转导途径与PKC信号途径是否存在相互应答。方法用PKC特异性抑制剂Bis-1预先处理HL-60细胞再用钙离子载体A23187处理,比较其与单用A23187处理的HL-60细胞在形态学、免疫表型及刺激T细胞增殖能力方面的差异。结果单用A23187处理的HL-60细胞培养36 h后细胞出现树状突起,DC特异性标记CD83、CD80、CD86表达阳性率分别为(28.97±4.05)%(、19.10±5.46)%、(41.03±6.43)%;用PKC特异性抑制剂Bis-1预先处理再予A23187处理36h的HL-60细胞其形态、免疫表型及刺激T淋巴细胞增殖的能力均较单用A23187的HL-60细胞受到不同程度的抑制,细胞表面DC特异抗原CD83、CD80、CD86分子的阳性表达率分别降为(13.23±2.15)%、(9.70±1.69)%、(23.37±7.50)%,与单用A23187处理的HL-60细胞比较,差异有统计学意义(P均<0.05)。结论 PKC特异性抑制剂Bis-1在A23187诱导HL-60细胞分化为DC的过程中起抑制作用,表明PKC信号路径参与A23187诱导HL-60白血病细胞分化为DC的过程。
Objective To investigate the role of intracellular PKC signaling pathway in the differentiation of HL-60 cells into dendritic cells (DCs) induced by A23187 using Bis-1, a specific inhibitor of protein kinase C (PKC) Calcium ion signal transduction pathway and PKC signaling pathway exist whether there is mutual response. Methods HL-60 cells were pretreated with PKC specific inhibitor Bis-1 and treated with calcium ionophore A23187. The morphological and immunophenotype of HL-60 cells treated with A23187 and the ability to stimulate T cell proliferation were compared The difference. Results The dendrites of HL-60 cells treated with A23187 for 36 h showed that the positive rates of DC-specific markers CD83, CD80 and CD86 were (28.97 ± 4.05)% (19.10 ± 5.46)% and ± 6.43)%. HL-60 cells pretreated with PKC-specific inhibitor Bis-1 and treated with A23187 for 36 hours had higher cell morphology, immunophenotype and ability to stimulate T lymphocyte proliferation than HL-60 cells treated with A23187 alone (13.23 ± 2.15)%, (9.70 ± 1.69)%, (23.37 ± 7.50)%, respectively. Compared with A23187 treatment alone, the positive rates of CD83, CD80 and CD86 on the cell surface were reduced to Of HL-60 cells, the difference was statistically significant (P all <0.05). Conclusions Bis-1, a specific inhibitor of PKC, plays an inhibitory role in the differentiation of HL-60 cells to DC induced by A23187, indicating that PKC signaling pathway is involved in the differentiation of HL-60 leukemia cells into DCs induced by A23187.