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为研究饲料中添加鱼油和豆油对中华绒螯蟹(Eriocheir sinensis)成蟹生长、免疫、代谢和耐低氧性能的影响,配制了添加不同比例鱼油和豆油的3种蟹用饲料,添加3%鱼油饲料组、3%豆油饲料组、3%鱼油和豆油混合组(1∶1,质量比),将其分别投喂中华绒螯蟹115 d后测量蟹体重、壳长和壳宽的变化,再将其放入溶解氧(dissolved oxygen,DO)为(9.06±0.06)mg/L和(2.57±0.44)mg/L的水体中,测定其免疫、代谢指标及耐低氧性能的变化。结果发现:投喂添加3种不同油脂饲料的中华绒螯蟹各组间体重无显著性差异;低氧胁迫对中华绒螯蟹代谢指标影响较大;添加鱼油和豆油混合油饲料组中华绒螯蟹血细胞密度、血蓝蛋白含量及超氧化物歧化酶、酸性和碱性磷酸酶、乳酸脱氢酶的活性都为最高,说明鱼油与豆油混合添加对中华绒螯蟹免疫和抗氧化能力有促进作用,并增加其耐低氧能力。
In order to study the effects of adding fish oil and soybean oil to feed on the growth, immunity, metabolism and hypoxia tolerance of crab Eriocheir sinensis, three kinds of crab feed with different proportions of fish oil and soybean oil were prepared, and 3% Fish feed group, 3% soybean oil diet group, 3% fish oil and soybean oil mixed group (1: 1, mass ratio) were fed 115 days after the mitten crab weight, shell length and shell width changes, Then the cells were placed in water with dissolved oxygen (DO) of (9.06 ± 0.06) mg / L and (2.57 ± 0.44) mg / L, and the changes of immunity, metabolism and hypoxia tolerance were measured. The results showed that there was no significant difference in the body weight of the Chinese mitten crab (Eriocheir sinensis) fed with 3 kinds of different fat diets; the hypoxia stress had a significant effect on the metabolic index of Eriocheir sinensis; Crab blood cell density, hemocyanin content and superoxide dismutase, acid and alkaline phosphatase, lactate dehydrogenase activity are the highest, indicating that fish oil and soybean oil mixed to enhance immunity and antioxidant capacity of Chinese mitten crab Role, and increase its resistance to hypoxia.