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Influences of PGR, sucrose, and ammonium nitrate on in vitro flowering and fruiting from cotyledon explants of P.frutescens were studied. The regenerated shoots at 2-4 cm from cotyledon explants on MS medium supplemented with0.5 mg L-1 BA and 1.0 mg L-1 IAA were excised and transferred to MS medium containing 30 g L-1 sucrose, 8.25 g L-1ammonium nitrate, and 1.0 mg L-1 BA. Following 40 d of culture, 86.2% of them flowered and set seeds. These seeds weregerminable and developed into flowering plants in the fields. This study provides a simple system for rapid breeding ofP. frutescens and studying the physiological mechanism of flowering of plants.
Influences of PGR, sucrose, and ammonium nitrate on in vitro flowering and fruiting from cotyledon explants of P. frutescens were studied. The regenerated shoots at 2-4 cm from cotyledon explants on MS medium supplemented with 0.5 mg L-1 BA and 1.0 mg L-1 IAA were excised and transferred to MS medium containing 30 g L-1 sucrose, 8.25 g L-1monium nitrate, and 1.0 mg L- 1 BA. Following 40 d of culture, 86.2% of them flowered and set seeds. These seeds were germinated and developed into flowering plants in the fields. This study provides a simple system for rapid breeding of P. frutescens and studying the physiological mechanism of flowering of plants.