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制备了二茂铁(Fc)修饰碳糊电极(Fc/CPE)并采用扫描电子显微镜(SEM)和电子能量散射谱(EDS)技术观察其表面形貌和分析其组成。采用循环伏安法(CV)和计时电量法(CC)研究了维生素K3(VK3)在该修饰电极上的电化学行为并计算了电极过程的动力学参数。Fc/CPE对VK3的电化学氧化还原具有良好的催化作用,VK3在该修饰电极上的电极反应为两电子、两质子参加的受扩散控制的可逆过程。采用差分脉冲伏安法(DPV)测得催化还原峰电流与VK3浓度在2.56×10-4～2.18×10-6mol/L范围内呈线性关系,检出限为1.6×10-7mol/L。方法可用于市售维生素K3注射液中VK3含量的测定。
Ferrocene (Fc) modified carbon paste electrode (Fc / CPE) was prepared and its surface morphology was observed by scanning electron microscopy (SEM) and electron energy dispersive spectroscopy (EDS) The electrochemical behavior of vitamin K3 (VK3) on the modified electrode was studied by cyclic voltammetry (CV) and chronocoulometry (CC), and the kinetic parameters of the electrode were calculated. Fc / CPE has a good catalytic effect on electrochemical redox reaction of VK3. The electrode reaction of VK3 on this modified electrode is a reversible diffusion-controlled process involving two electrons and two protons. The catalytic reduction peak current measured by differential pulse voltammetry (DPV) showed a linear relationship with the concentration of VK3 in the range of 2.56 × 10-4 ~ 2.18 × 10-6mol / L with a detection limit of 1.6 × 10-7mol / L. Method can be used for the determination of VK3 in vitamin K3 injection.