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应用外径约 0 .3mm的导管从大鼠颈外动脉逆行插管至颈内动脉 ,一直到距大脑中动脉 (MCA)起始处 2～ 3mm处 ,注射凝血酶诱导血栓形成。于手术后 3、6、12h观察行为障碍并评分 ,手术后 12h取脑观察Willis环、脑梗死体积及病理改变。结果表明 ,该模型行为障碍和脑梗死体积较为恒定 ,观察Willis环可见MCA起始部血栓形成 ,所形成的血栓是混合血栓 ,脑梗死体积与本研究室应用线栓法建立的大鼠MCA缺血模型的梗死体积无明显差异。该模型操作简便 ,缺血效果可靠 ,梗死部位恒定 ,而且较接近人类脑血栓形成的临床特点 ,适用于评价溶栓治疗效果的实验研究
A catheter with an external diameter of about 0.3 mm was cannulated retrogradely from the rat external carotid artery to the internal carotid artery until a distance of 2 to 3 mm from the beginning of the middle cerebral artery (MCA). Thrombin injection induced thrombus formation. Behavioral disorders were observed at 3, 6, and 12 hours after surgery. The brains were harvested at 12 hours after surgery to observe the volume and pathological changes of Willis ring and cerebral infarction. The results showed that the model of behavioral disorders and cerebral infarction volume is relatively constant, the Willis ring can be seen starting MCA thrombosis, the formation of thrombus is a mixed thrombus, cerebral infarction volume and the establishment of the laboratory thread occlusion MCA lack There was no significant difference in the infarct volume in the blood model. The model is simple and easy to operate, with reliable ischemic effect, constant infarct size, and close to the clinical features of human cerebral thrombosis, and is suitable for the experimental study on evaluating the therapeutic effect of thrombolytic therapy