论文部分内容阅读
观察肾小球系膜细胞（ＭＣ）的转化生长因子（ＴＧＦ－β）的基因表达，以及内皮素（ＥＴ）对ＴＧＦ－β基因表达的影响。方法应用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）及半定量ＲＴ－ＰＣＲ方法对体外培养的大鼠ＭＣ进行了ＴＧＦ－β基因表达的定量检测。结果ＭＣ有ＴＧＦ－β的基因表达，并且ＥＴ对ＴＧＦ－β的基因表达有上调作用。结论ＴＧＦ－β是ＭＣ的又一个自分泌因子，ＥＴ和ＴＧＦ－β可能有协同作用而引起ＭＣ增生和系膜基质（ＭＭ）的增多，在肾小球肾炎和肾小球硬化的病理过程中，具有一定意义
To observe the gene expression of transforming growth factor (TGF-β) in glomerular mesangial cells (MC) and the effect of endothelin (ET) on TGF-β gene expression. Methods Quantitative detection of TGF-β gene expression in rat MC cultured in vitro was performed by reverse transcription-polymerase chain reaction (RT-PCR) and semi-quantitative RT-PCR. Results MC had TGF-β gene expression, and ET up-regulated the gene expression of TGF-β. Conclusion TGF-β is another autocrine factor of MC. ET and TGF-β may have a synergistic effect and cause MC hyperplasia and mesangial matrix (MM) increase. In the pathological process of glomerulonephritis and glomerulosclerosis , Has a certain meaning