论文部分内容阅读
目的了解福建省2011年病毒性脑炎暴发的病原特点及福建省近10年Echo30分子流行病学特征。方法应用细胞培养法获得病毒株,微量血清中和试验或RT-PCR序列分析鉴定Echo30血清型,提取病毒RNA核酸,逆转录为cD-NA,分段扩增VP1基因序列全长,测定并分析扩增产物的序列。结果 2011年4月1日-6月2日,从福建省4个县(市)区病毒性脑炎病例标本中分离的病毒经鉴定均为Echo30;VP1基因序列分析显示,泉州市安溪和德化两地流行株高度同源(核苷酸与氨基酸序列同源性分别为99.0%和99.6%),同福安和厦门流行株(核苷酸与氨基酸序列同源性分别为98.5%和100%)核苷酸差异约9%,3个位点氨基酸出现了变异。近10年福建省Echo30分子流行病学分析显示,2011年泉州流行株并非由2005年当地流行株进化而来,而与2008年河南省分离株(HQ625646-Henan/04/2008)同源性最高;近10年福建省Echo30存在多基因型、多谱系的同时传播,并在2006年发现了两株第III新基因型病毒。结论造成2011年福建省病毒性脑炎暴发的病原为Echo30,且至少存在两条不同传播链病毒同时流行。Echo30福建株呈多基因型、多谱系分布流行特点。
Objective To understand the etiological characteristics of encephalitis outbreaks in Fujian Province in 2011 and the molecular epidemiology of Echo30 in Fujian Province in recent 10 years. Methods The virus strains were obtained by cell culture method. The serotypes of Echo30 were identified by micro-serum neutralization assay or RT-PCR analysis. The viral RNA was extracted and reverse transcribed into cD-NA. The full-length VP1 gene was amplified and sequenced. The sequence of the amplification product. Results From April 1, 2011 to June 2, 2011, Echo30 viruses were isolated from the virus encephalitis cases in 4 counties of Fujian Province. The sequence analysis of VP1 gene showed that Anxi Hede The two strains were highly homologous (nucleotide and amino acid sequence homologies were 99.0% and 99.6%, respectively), and Fu-An and Xiamen Epidemic strains (nucleotide and amino acid sequence homologies were 98.5% and 100% ) Nucleotide difference of about 9%, 3 amino acid mutation. The molecular epidemiological analysis of Echo30 in Fujian Province in recent 10 years showed that the epidemic strains of Quanzhou in 2011 did not evolve from the endemic strains in 2005 but the highest homology with the isolates from Henan Province in 2008 (HQ625646-Henan / 04/2008) In the recent 10 years, there were multiple genotypes and multi-lineages of Echo30 in Fujian Province. At the same time, two new genotype III viruses were discovered in 2006. Conclusion The etiological agent causing encephalitis outbreak in Fujian province in 2011 is Echo30, and at least two different transmission chain viruses exist simultaneously. Echo30 Fujian strains were multi-genotype, multi-lineage distribution of epidemic characteristics.