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目的采用聚合酶链式反应-限制性酶切片段长度多态性(PCR-RFLP)技术及病例对照研究,探讨β3肾上腺素能受体(β3AR)基因Trp64Arg突变与中国人2型糖尿病(DM)的相关性。方法选择无亲缘关系2型DM患者151例,作为2型DM组。另选80例体检健康成人,作为正常对照组。对2型DM组与对照组间的临床生化指标及β3ARTrp64Arg等位基因频率与基因型频率进行比较。结果2型DM组β3AR64Arg等位基因频率为17%,β3ARTrp/Arg基因型频率为32.5%,对照组β3AR64Arg等位基因频率为15%,Trp/Arg基因型频率为30%,二组之间无明显差异(P>0.05)。结论β3AR基因Trp64Arg突变在杂合子状态可能不是中国人2型DM的主要危险因素。
Objective To investigate the relationship between Trp64Arg mutation of β3AR gene and Chinese type 2 diabetes mellitus (DM) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and case-control study. Relevance. Methods 151 unrelated patients with type 2 DM were selected as type 2 DM patients. Another 80 cases of healthy adults, as a normal control group. The clinical biochemical indexes and the frequency of β3ARP64Arg alleles and genotype frequencies between type 2 DM group and control group were compared. Results The frequency of β3AR64Arg allele in type 2 diabetes mellitus was 17%, the frequency of β3 ARPAR / Arg genotype was 32.5%, the frequency of β3AR64Arg allele in control group was 15%, and the frequency of Trp / Arg genotype was 30% Significant difference (P> 0.05). Conclusion The mutation of Trp64Arg of β3AR gene in heterozygous state may not be the main risk factor of type 2 DM in Chinese.