论文部分内容阅读
了解不同倍性间杂交后代的倍性分离及其遗传分化规律对定向育种、多倍体种质创制及理论创新具有重要意义。利用流式细胞仪及AFLP分子标记检测不同倍性的8个中华猕猴桃杂交组合群体子代倍性分离及遗传分化规律。结果表明:六倍体母本‘新观2号’与二倍体父本‘红阳’雄系和‘桂海’雄系杂交子代群体创制出新的倍性个体(3x、4x、5x、7x、8x),且杂交组合群体的遗传多样性(H:0.4166和0.4305)、Shannon信息指数(I:0.5833和0.6053)、多态位点百分率(P:91.18%和92.63%)均显著高于其他组合;而倍性差较小的4个杂交组合[4x(♀)×6x(♂)、4x(♀)×2x(♂)、4x(♀)×2x(♂)和2x(♀)×4x(♂)],其基因多样性(0.3174、0.3346、0.3276和0.3198)、Shannon信息指数(0.4269、0.4568、0.4420和0.4445)、多态位点百分率(71.27%、76.14%、74.72%和75.84%)水平相对较低,且创制出奇数倍性个体(5x、3x、3x)。进一步遗传分析表明,除6号杂交组合[4x(♀)×2x(♂)]外,绝大部分杂交组合的后代群体与母本具有更近的遗传距离。
It is of great significance to understand the ploidy segregation and its genetic differentiation among the progenies of different ploidy crossbreeds for directional breeding, polyploid germplasm creation and theoretical innovation. Flow cytometry and AFLP molecular markers were used to detect ploidy segregation and genetic differentiation of offspring of eight Chinese kiwifruit hybrid combinations with different ploidy. The results showed that a new ploidy individuals (3x, 4x, 5x) were created by the hybrid progeny of hexaploid female parent Xin’an 2 and diploid male parents ’Hongyang’ and ’Guihai’ , 7x, 8x). The genetic diversity (H: 0.4166 and 0.4305), Shannon’s information index (I: 0.5833 and 0.6053), the percentage of polymorphic loci (P: 91.18% and 92.63% (4x (♀) × 6x (♂), 4x (♀) × 2x (♂), 4x (♀) × 2x (♂), and 2x (♀) × Shannon’s information index (0.4269,0.4568,0.4420 and 0.4445). The percentage of polymorphic loci (71.27%, 76.14%, 74.72% and 75.84%, respectively) ) Is relatively low and creates odd-numbered ploidy individuals (5x, 3x, 3x). Further genetic analysis showed that most of the progeny populations of the crosses had a closer genetic distance from the female than the 6th cross [4x (♀) × 2x (♂)].