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在pH 1.0～ 1.5的酸性介质中 ,蛋白质与Ba 偶氮氯膦Ⅲ络合物发生作用 ,导致络合物溶液褪色 ,其最大吸收波长处吸光度的降低值与蛋白质的浓度成正比。基于此建立了以Ba 偶氮氯膦Ⅲ络合物为光谱探针 ,分光光度测定蛋白质含量的新方法。该法灵敏度高 ,选择性好。蛋白质 (以牛血清白蛋白为例 )的浓度在 0～ 4 0mg/L范围内服从比尔定律 ,表观摩尔吸光系数ε656=6 .4 7× 10 5L·mol-1·cm-1。生物体内常见物质均不产生干扰 ,直接应用于人血清样品中蛋白质总量的测定 ,结果满意
In an acidic medium at pH 1.0-1.5, the protein interacts with the Ba-chlorophosphonazo complex, resulting in the fading of the complex solution. The decrease in absorbance at the maximum absorption wavelength is proportional to the protein concentration. Based on this, a new method for spectrophotometric determination of protein content using Ba-chlorophosphonazo complex as spectral probe was established. The method of high sensitivity, good selectivity. Beer’s law was obeyed in the range of 0-4 0 mg / L for protein (bovine serum albumin). The apparent molar absorptivity ε656 = 6.47 × 10 5 L · mol -1 · cm -1. The common substances in the body do not produce interference, directly applied to the determination of the total protein in human serum samples, the results are satisfactory