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目的:建立测定茵柏益肝汤中有效成分芹菜素的HPLC方法,考察药材不同配伍对芹菜素溶出率的影响。方法:采用RP-HPLC方法测定茵柏益肝汤不同配伍水煎液浸膏粉中芹菜素的含量,色谱柱为Zorbax Extend-C18柱,流动相:甲醇-0.2%磷酸溶液(52∶48);流速:1.0 m L/min;检测波长:350 nm;柱温:40℃。结果:建立的茵柏益肝汤中芹菜素测定的HPLC方法准确、快速、重现性好;不同配伍对茵柏益肝汤中芹菜素含量影响显著。结论:茵陈、矮地茶能够提高茵柏益肝汤中芹菜素溶出率,金钱草能够降低二者对芹菜素的增溶作用。
OBJECTIVE: To establish an HPLC method for the determination of apigenin in Yinbaiyi Gan Tang, and to investigate the effect of different compatibility on the dissolution rate of apigenin. Methods: The content of apigenin in Yinbaiyigan decoction extract powder was determined by RP-HPLC. The column was Zorbax Extend-C18 column. The mobile phase consisted of methanol-0.2% phosphoric acid solution (52:48) ; Flow rate: 1.0 m L / min; detection wavelength: 350 nm; column temperature: 40 ° C. Results: The established HPLC method for determination of apigenin in Yinbaiyi Gan Tang was accurate, rapid and reproducible. The effect of different compatibility on the content of apigenin in Yinbaiyi Gan Tang was significant. Conclusions: Yinchen and shortecha tea can improve the dissolution rate of apigenin in Yinbaiyi Gan Tang, and the cheilitis can reduce the solubilization of apigenin.