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补髓生血冲剂是我院治疗再生障碍性贫血中药复方,临床应用已十多年,为了进一步了解本方药对机体造血功能的调控作用,我们用内、外源性脾结节法和体内扩散盒半固体培养法观察了补髓生血冲剂对造血干细胞(CFU-S)和粒系祖细胞(CFU-D)的影响。 1 材料和方法 1.1 动物:健康昆明系封闭群小鼠,雌雄各半,6～10周龄,体重20～25g,黑龙江中医药大学动物实验中心提供。 1.2 补髓生血冲剂药物组成:补髓生血冲剂Ⅰ号:生
Bupeng Shengxue Granules is a traditional Chinese medicine compound for the treatment of aplastic anemia in our hospital. It has been used clinically for more than ten years. To further understand the regulatory effect of this prescription on hematopoietic function, we use internal and exogenous splenic nodules and diffusion kits. Semi-solid culture method was used to observe the effect of BJS on CFU-S and CFU-D. 1 Materials and Methods 1.1 Animals: Healthy Kunming mice were divided into groups of males and females, 6 to 10 weeks old, weighing 20 to 25 g, provided by Animal Experimental Center of Heilongjiang University of Chinese Medicine. 1.2 Bushengshengxue granules Drug composition: Bushengshengxue Granule I: Health