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目的:探讨游离脂肪酸(FFAs)对人牙周膜成纤维细胞增殖的影响,研究游离脂肪酸在代谢综合征患者牙周病发病机制中的作用。方法:选用在牙周组织修复中起主要作用的人牙周膜成纤维细胞进行体外培养,对照组加入不含胎牛血清的DMEM,实验组分别加入不同浓度的游离脂肪酸进行刺激,在刺激24h-72h后,采用四甲基偶氮唑蓝比色(MTT)法检测人牙周膜成纤维细胞的增殖情况。结果:与对照组相比,游离脂肪酸可以抑制人牙周膜成纤维细胞的生长增殖(P<0.01),并且这种抑制作用具有浓度和时间依赖性,以培养72h后抑制作用最为明显(P<0.01)。结论:游离脂肪酸可以抑制牙周膜成纤维细胞的增殖,降低代谢综合征患者牙周组织的的修复能力,从而导致或加重牙周病的发生或发展。
Objective: To investigate the effects of free fatty acids (FFAs) on the proliferation of human periodontal ligament fibroblasts and the role of free fatty acids in the pathogenesis of periodontal disease in patients with metabolic syndrome. Methods: Human periodontal ligament fibroblasts, which play a major role in periodontal tissue repair, were cultured in vitro. DMEM without fetal bovine serum was added into the control group. Different concentrations of free fatty acids were added to the experimental group to stimulate. After 24h After -72h, the proliferation of human periodontal ligament fibroblasts was detected by MTT assay. Results: Compared with the control group, free fatty acids could inhibit the proliferation of human periodontal ligament fibroblasts (P <0.01), and the inhibitory effect was concentration-dependent and time-dependent <0.01). Conclusion: Free fatty acids can inhibit the proliferation of periodontal ligament fibroblasts and reduce the repair capacity of periodontal tissues in patients with metabolic syndrome, resulting in or aggravating the occurrence or development of periodontal disease.