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目的分析P21蛋白在胰腺癌和癌旁组织中的表达及与预后的关系。方法共90例胰腺癌患者纳入研究,每例均有完整的临床资料及对应的癌组织和癌旁组织标本,采用免疫组化技术进行芯片染色。分析免疫组化染色结果中P21的表达与胰腺癌临床病理特征的关系,并进行生存分析,探讨P21对预后的判断价值。结果 90例胰腺癌组织中P21阳性表达率为48.9%。P21阳性表达与肿瘤大小、淋巴结转移、临床分期及细胞核增殖抗原(Ki-67)表达等相关(P<0.05)。P21阳性组患者的总生存期(Overall survival,OS)低于P21阴性组患者(P=0.022)。Cox多因素分析显示胰腺癌和癌旁组织中P21的阳性表达以及胰腺癌组织中Ki-67阳性表达为患者不良预后的独立影响因素。结论胰腺癌组织中P21阳性表达预示胰腺癌的不良预后,Ki-67与P21在胰腺癌组织中同时阳性表达或胰腺癌癌旁组织中P21阳性表达可增加不良预后风险。
Objective To analyze the expression of P21 protein in pancreatic cancer and paracancerous tissues and its relationship with prognosis. METHODS: A total of 90 patients with pancreatic cancer were enrolled in the study. Each patient had complete clinical data and corresponding cancer tissue and paracancerous tissue samples. Immunohistochemistry was used for chip staining. To analyze the relationship between the expression of P21 and the clinicopathological features of pancreatic cancer in immunohistochemical staining, and to analyze the survival of P21 and evaluate the prognostic value of P21. Results The positive rate of P21 in pancreatic cancer tissues was 48.9%. The positive expression of P21 was correlated with tumor size, lymph node metastasis, clinical stage and expression of Ki-67 (P <0.05). The overall survival (OS) of P21-positive patients was lower than that of P21-negative patients (P = 0.022). Cox multivariate analysis showed that the positive expression of P21 in pancreatic cancer and paracancerous tissues and the positive expression of Ki-67 in pancreatic cancer tissues were independent prognostic factors. Conclusions The positive expression of P21 in pancreatic cancer indicates the poor prognosis of pancreatic cancer. The positive expression of Ki-67 and P21 in pancreatic cancer and the positive expression of P21 in pancreatic cancer may increase the risk of adverse prognosis.