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目的研究川芎嗪对IL-1β诱导的兔关节软骨细胞损伤的影响。方法体外分离培养兔关节软骨细胞,并以其为实验模型;将实验分为正常对照组、白介素组、川芎嗪+白介素组;MTT法检测细胞增殖情况;Real-time PCR检测MMP-1、MMP-13和TIMP-1的mRNA表达量。结果 MTT检测结果显示,川芎嗪+白介素组细胞增殖情况显著高于正常对照组和白介素组(P<0.05);Real-time PCR结果显示,川芎嗪+白介素组MMP-1和MMP-13的mRNA表达量显著低于白介素组(P<0.05),而TIMP-1的mRNA表达量则显著高于白介素组(P<0.05)。结论川芎嗪可抑制IL-1β对软骨细胞的炎症损伤,具有保护软骨细胞,延缓软骨基质降解的作用,临床上可作为OA的治疗用药。
Objective To study the effect of ligustrazine on IL-1β induced rabbit articular chondrocyte injury. Methods Rabbit articular chondrocytes were isolated and cultured in vitro. The experiment was divided into normal control group, interleukin group, ligustrazine + interleukin group, MTT assay and Real-time PCR to detect the expression of MMP-1, MMP -13 and TIMP-1 mRNA expression levels. Results The results of MTT assay showed that the proliferation of ligustrazine + interleukin group was significantly higher than that of normal control group and interleukin (P <0.05). The results of Real-time PCR showed that the mRNA of MMP-1 and MMP-13 The expression of TIMP-1 was significantly lower than that of interleukin (P <0.05), while the mRNA expression of TIMP-1 was significantly higher than that of interleukin (P <0.05). Conclusion Tetramethylpyrazine can inhibit IL-1βin inflammatory injury of chondrocytes, which has the function of protecting chondrocytes and delaying the degradation of cartilage matrix, and can be used clinically as a therapeutic drug for OA.