论文部分内容阅读
目的 观察大鼠肝硬化门脉高压形成过程中外周血浆内皮素-1(ET-1)和一氧化氮(NO)的动态变化,以探讨两种物质在门脉高压高动力循环中的作用。方法 肝硬化门脉高压组大鼠模型用四氯化碳加乙醇制备,对照组只注射等量橄榄油。放免法检测血浆ET-1水平,硝酸还原酶法检测NO,观察两者在不同时期的变化。结果 模型制备过程中的第0、2和6周未见两组动物之间存在NO和ET-1水平的显著性差异,第10周时有ET-1水平的降低和NO水平的显著升高,同时有门脉压力的增高和体循环平均动脉压的降低。结论ET-1水平的降低和NO水平的增高是引起肝硬化门脉高压高动力循环产生的重要原因。
Objective To observe the dynamic changes of plasma endothelin-1 (ET-1) and nitric oxide (NO) in the pathogenesis of portal hypertension in rats with cirrhosis and to explore the roles of these two substances in portal hypertension hyperdynamic circulation. Methods The rat model of cirrhosis and portal hypertension was prepared with carbon tetrachloride and ethanol, while the control group was injected only with the same amount of olive oil. The level of plasma ET-1 was detected by radioimmunoassay and the level of NO was detected by nitrate reductase method. Results There was no significant difference in the levels of NO and ET-1 between the two groups at 0, 2 and 6 weeks after model preparation. There was a significant decrease of ET-1 and NO at week 10 , While increased portal pressure and systemic mean arterial pressure decreased. Conclusion The decrease of ET-1 level and the increase of NO level are the important reasons for the hyperdynamic circulation of cirrhotic patients with portal hypertension.