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目的研究侧脑室注射orexin-A对氯胺酮-咪达唑仑麻醉大鼠的促醒作用。方法腹腔注射氯胺酮(75mg/kg)和咪达唑仑(5mg/kg)麻醉大鼠后,侧脑室注射不同剂量orexin-A,以脑电δ波比例评价麻醉深度,以大鼠翻正反射消失(LRR)持续时间评价麻醉时间,以共济失调期评价运动功能恢复。结果同对照组相比,高剂量orexin-A治疗组(4nmol)麻醉大鼠脑电δ波比例明显降低(P<0.01),LRR持续时间和共济失调期明显缩短(P<0.01);而低剂量orexin-A治疗组(1nmol)无明显变化(P>0.05)。结论侧脑室注射orexin-A可使麻醉深度变浅,麻醉时间缩短,并可促进麻醉后运动功能恢复,从而发挥了对麻醉的促醒作用。
Objective To investigate the effects of intracerebroventricular injection of orexin-A on ketamine-midazolam anesthesia in rats. Methods After intraperitoneal injection of ketamine (75 mg / kg) and midazolam (5 mg / kg), different doses of orexin-A were injected into the lateral ventricle. The depth of anesthesia was evaluated by EEG wave ratio, (LRR) duration to evaluate anesthesia time and motor function recovery with ataxia. Results Compared with the control group, the proportion of 5-wave EEG in high-dose orexin-A treated group (4 nmol) was significantly decreased (P <0.01), and the duration of LRR and ataxia were significantly shorter (P <0.01) Low-dose orexin-A treatment group (1nmol) no significant change (P> 0.05). Conclusion Intra-ventricle injection of orexin-A can make the depth of anesthesia shallow, the anesthesia time shortened, and promote the recovery of motor function after anesthesia, which play a role in the awakening of anesthesia.