遥感技术使大面积监测植物长势及估测化学组成有了可能，高分辩力地物光谱仪应用于简单、快速、非破坏性地估测植物冠层化学组成的前景正引起越来越多的关注．本文通过田间试验及室内分析研究了氮肥水平对水稻叶片及冠层反射光谱特性的影响，氮素营养对从可见光至近红外较大范围水稻反射光谱特性有较大影响，随着氮素营养水平的提高，叶片及冠层可见光波段的光谱反射率下降，而近红外波段则增加，经两样本ｔ检验统计分析，在５４０，６８０ｎｍ和７４０～１０７０ｎｍ波段处对氮素营养水平最为敏感，而且各氮营养水平之间的反射率呈显著或极显著的差异．水稻叶片氮含量与冠层和叶片光谱变量呈极显著或显著的相关性，这表明利用光谱测试简单、快速、非破坏性地估测水稻氮素营养水平是可行的． Remote sensing technology has made it possible to monitor plant growth over large areas and to assess chemical composition, and the prospect of using high-resolution spectroscopy in the simple, rapid and non-destructive assessment of the canopy chemical composition is attracting more and more attention . In this paper, field experiments and laboratory analyzes were conducted to study the effect of nitrogen level on the reflectance spectra of rice leaves and canopy. Nitrogen nutrition had a significant effect on the reflectance spectra of rice from visible light to near infrared range. With the increase of nitrogen nutrition level The spectral reflectance in the visible light range of leaves and canopy decreased while that in the near-infrared range increased. The t-test of two samples showed that the spectral reflectance was the most sensitive in the bands of 540, 680nm and 740 ~ 1070nm, and the contents of nitrogen Reflectance between nutrient levels showed significant or very significant differences. Rice leaf nitrogen content and canopy and leaf spectral variables were significant or significant correlation, indicating that the use of spectral test simple, rapid and non-destructive assessment of rice nitrogen nutrition level is feasible.