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1989─1992年间对杭州等地一串红(Salviasplendens)花叶病的发生及其病原进行了系统研究,从病株上分离得到的17个病毒分离物,经生物学和血清学反应测定均符合黄瓜花叶病毒(CMV)的特点。病毒分离物M-22不能经由桃蚜传播使黄苗榆等供试寄主发病。用0.5mol/L磷酸钾盐缓冲液对M-22进行提纯,提纯病毒经醋酸铀负染,测定其粒子大小为30.2nm。SDS-聚丙烯酰胺凝胶电泳测定M-22的外壳蛋白分子量约为28kD,经琼脂双扩散测定,M-22与几个已知CMV抗血清均有强阳性反应,M-22与一个番茄不孕病毒(TAV)抗血清有阳性反应,但其抗血清与供试TAV抗原无阳性反应。ELIsA反应测定结果表明,M-22与一个来自于十字花科大白菜上的CMV分离物和一个来自于前科烟草上的CMV分离物,在血清学关系上有明显差异。根据实验结果,作者认为目前在杭州等地侵染一串红的病原病毒主要是CMV,侵染一串红的CMV分离物M-22可能是一个独立的血清型.
From 1989 to 1992, the pathogenesis and pathogenesis of Salviasplendens mosaic disease in Hangzhou and other places were systematically studied. The 17 virus isolates isolated from diseased plants were determined by both biological and serological tests Cucumber mosaic virus (CMV) features. Virus isolate M-22 can not spread through the green peach aphid such as test host disease. M-22 was purified with 0.5mol / L potassium phosphate buffer and the virus was negatively stained with uranyl acetate. The particle size was determined to be 30.2nm. The molecular weight of the coat protein of M-22 was about 28kD by SDS-polyacrylamide gel electrophoresis. M-22 had strong positive reaction with several known CMV antiserums by agar double diffusion assay. M-22 and one tomato did not TAV antisera reacted positively but no antisera reacted positively with the tested TAV antigen. The results of ELIsA reaction assay showed that there was a significant serological relationship between M-22 and one CMV isolate from Brassicaceae cabbage and one CMV isolate from the same strain. According to the experimental results, the authors believe that currently infected with a bunch of red pathogenic viruses in Hangzhou and other places is mainly CMV, infected with a bunch of red CMV isolate M-22 may be an independent serotype.