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目的 探讨阿霉素磁性蛋白微球联合外磁场体内外抑制肿瘤生长的机制。方法 Wistar大白鼠 3 2只 ,用MTT法检测阿霉素磁性蛋白微球对Walker 2 5 6细胞的抑制率及半数抑制剂量 ;观察其体内靶向治疗敏感细胞鼠种植性胃肿瘤的疗效。结果 阿霉素磁性蛋白微球与游离阿霉素体外对肿瘤细胞的生长抑制率相似 (P >0 .0 5 ) ,联合外磁场后其抑制率作用明显增强 (P <0 .0 1)。靶向组对种植瘤的抑制率达 82 .5 2 % (P <0 .0 1) ,荷瘤动物生成时间明显延长 ,延长率达 2 86.3 8% (P <0 .0 1)。结论 阿霉素磁性蛋白微球体内外仍具有抑制敏感肿瘤细胞生长的能力 ,联合磁场可发挥靶向、高效的抗瘤作用。
Objective To investigate the mechanism of adriamycin magnetic protein microspheres combined with external magnetic field to inhibit tumor growth in vitro and in vivo. Methods The Wistar rats were used in 32 rats. The inhibitory rate and half inhibition of Walker 256 cells were measured by MTT assay. The therapeutic effect of adriamycin magnetic protein microspheres on Walker 256 cells was observed. Results The proliferation inhibition rate of adriamycin magnetic protein microspheres and free doxorubicin was similar to that of tumor cells in vitro (P > 0.05), and the inhibition effect was significantly enhanced after combined external magnetic field (P < 0.01). The inhibitory rate of the tumor targeting group was 82.52% (P < 0.01), and the tumor-bearing animal generation time was significantly prolonged. The prolongation rate was 2 86.3 8% (P <0.01). Conclusion The adriamycin magnetic protein microspheres still have the ability to inhibit the growth of sensitive tumor cells in vitro and in vivo. The combined magnetic field can exert a targeted and highly effective anti-tumor effect.