Biomarkers of paleolake deposits from Qarhan Salt Lake in Qaidam Basin,northwest China were sys-tematically analyzed and the A―C series compounds of branched aliphatic alkanes with quaternary substituted carbon atom(BAQCs) were identified. The homologous distinguished three series,A―C,were identified as 5,5-diethylalkanes,6,6-diethylalkanes and 5-butyl,5-ethylalkanes series,and their relative abundance was A > B > C. Series A and C were characterized by odd carbon numbers,whereas series B was characterized by even carbon numbers. The high values of series A corresponded with the high values of series B and C. Therefore,it can be concluded that series A,B and C possess a similar biological origin. The abundance of series A was relatively low in the lower part of the section compared with that in the upper part,implying that these series originated from bacteria and/or algae more prevalent in fresh-mesohaline water,and such kinds of bacteria and/or algae are most likely to be thermophilous species. The A25/nC25 ratio differences in the section show that such branched aliphatic alkanes can be treated as one kind of environmental change proxy for paleolake evolution and may provide important information for the climate reconstruction of the Late Pleistocene. Biomarkers of paleolake deposits from Qaritan Salt Lake in Qaidam Basin, northwest China were sys-tematically analyzed and the A-C series compounds of branched aliphatic alkanes with quaternary substituted carbon atoms (BAQCs) were identified. The homologous distinguished three series, A-C , were identified as 5,5-diethylalkanes, 6,6-diethylalkanes and 5-butyl, 5-ethylalkanes series, and their relative abundance was A> B> C. Series A and C were characterized by odd carbon numbers, The high values ​​of series A are stacked with the high values ​​of series B and C. Therefore, it can be said that series A, B and C possess similar similar to origin. The abundance of series A was relatively similar low in the lower part of the section compared with that in the upper part, implying that these series originated from bacteria and / or algae more prevalent in fresh-mesohaline water, and such kinds of bacteria and / or algae are most likely to be thermop The A25 / nC25 ratio differences in the section show that such branched aliphatic alkanes can be treated as one kind of environmental change proxy for paleolake evolution and may provide important information for the climate reconstruction of the Late Pleistocene.