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目的:研究食管癌组织中VEGF-CmRNA和蛋白的表达及其与食管癌临床病理特征关系,探讨VEGF-C在食管癌淋巴转移中的作用。方法:采用逆转录聚合酶链反应(RT-PCR)、免疫组织化学染色等方法,通过光镜和图像分析法检测VEGF-C在食管癌中的表达,分析其与食管癌临床病理参数关系。结果:食管癌VEGF-CmRNA的表达阳性率显著高于癌旁组织(P<0.05),在食管癌组织中存在VEGF-C蛋白的表达,VEGF-C的表达与患者的年龄、性别、临床病理学分级无关(P>0.05),而与淋巴结转移密切相关。结论:食管癌组织VEGF-C的表达与淋巴道的转移有关,VEGF-C检测可作为预测食管癌淋巴道转的指标之一。
Objective: To investigate the expression of VEGF-C mRNA and protein in esophageal carcinoma and its relationship with the clinicopathological features of esophageal cancer and to explore the role of VEGF-C in lymphatic metastasis of esophageal cancer. Methods: The expression of VEGF-C in esophageal cancer was detected by light microscopy and image analysis by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical staining. The relationship between VEGF-C and clinicopathological parameters was analyzed. Results: The positive rate of VEGF-C mRNA expression in esophageal cancer was significantly higher than that in paracancerous tissues (P <0.05). The expression of VEGF-C protein in esophageal cancer tissue was correlated with the age, sex, Neither grade nor grade (P> 0.05), but closely related to lymph node metastasis. Conclusion: The expression of VEGF-C in esophageal carcinoma is related to lymph node metastasis. VEGF-C can be used as a predictor of lymphatic transit in esophageal cancer.